SuperMag Carboxyl Beads, Combo Pack, any three (3) sizes from 50 nm to 200 nm

• Concentration: 10 mg/mL
• Storage Buffer: DI water, 0.05% NaN3, 0.01% tween 20
• Magnetic Content: ~80%
• Size: 50 nm-200 nm

SuperMag Carboxyl Beads are hydrophilic magnetic beads with carboxylic acid groups. These surface groups allow covalent amide bond formation to antibodies, proteins, peptides or other biomolecules via primary amine (NH2) groups. Oligonucleotides, antibodies or other ligands with amine groups can be easily coupled to the beads. Activation through carbodimide is required. With large surface area, excellent colloidal stability and unique surface coating, SuperMag Carboxyl Beads exhibit high binding capacity and low non-specific binding of protein or nucleic acids.

SuperMag Carboxyl Beads are also available in an easy-to-use kit format to enable researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic beads. With the engineered SuperMag Carboxyl Beads and the specifically formulated conjugation buffers, the resulting conjugates exhibit high binding capacity and low non-specific binding. The kit contains sufficient reagents & components for performing at least 4 conjugation reactions. Briefly, the magnetic beads are activated using carbodiimide and N-hydroxysuccinimide followed by conjugation to amino groups that are present on the target protein/ligand. The recommended protocol has been used to successfully conjugate bovine serum albumin, streptavidin or immunoglobulin to these magnetic beads.

Features:

• High Binding Capacity
• Significantly low non-specific binding to proteins and nucleic acid.
• Immediate and gentle coupling at room temperature and neutral to low pH
• Fast Magnetic Separation

Applications:

• CTC separation
• Cell separation
• Protein separation
• Bacteria separation
• Immunoassays