HiSur Amine Beads, 1 µm

HiSur Amine Beads are hydrophilic magnetic beads with amine groups. These surface groups allow covalent bond formation to antibodies, proteins, peptides or other biomolecules via primary amine, carboxy or thiol groups. Oligonucleotides, antibodies or other ligands with these groups can be easily coupled to the beads. Activation through EDC or Sulfo-SMCC is required. Attribute to their very large surface area (about three times larger than that of the MonoMag Amine Beads and unique surface coating, HiSur Amine Beads exhibit superior binding capacity and low non-specific binding of protein or nucleic acids.

Ocean NanoTech’s HiSur Amine Beads (1 µm) are also available in an easy-to-use kit format, allowing researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic beads. With the engineered HiSur Amine Beads (1 µm) and the specifically formulated conjugation buffers, the resulting conjugates exhibit superior binding capacity and low non-specific binding. The kit contains sufficient reagents & components for performing at least 5 conjugation reactions. Briefly, the magnetic beads are activated using SMCC (succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate) followed by conjugation to thiol groups that are present on the target protein/ligand. The recommended protocol has been used to successfully conjugate bovine serum albumin, streptavidin or immunoglobulin to these magnetic beads.

Features:

• Superior binding capacity
• Significantly low non-specific binding to proteins and nucleic acid
• Immediate and gentle coupling at room temperature and neutral to low pH
• Comparable performance with Sera Mag Amine Modified and Sera Mag SpeedBeads Amine Modified

Applications:

• Immunoprecipitation
• Nucleic acid isolation
• Assay development