SuperMag Amine Beads, 100 nm

Overview

• Concentration: 10 mg/mL
• Storage buffer: DI water, 0.05% NaN3, 0.01% tween 20
• Magnetic Content: ~80%
• Size: 50-200 nm

SuperMag Amine Beads are hydrophilic magnetic beads with amine groups. These surface groups allow covalent bond formation to antibodies, proteins, peptides or other biomolecules via primary amine, carboxy or thiol groups. Oligonucleotides, antibodies or other ligands with these groups can be easily coupled to the beads. Activation through EDC or Sulfo-SMCC is required. With large surface area, excellent colloidal stability and unique surface coating, the SuperMag Amine Beads exhibit high binding capacity and low non-specific binding of protein or nucleic acids.

Ocean NanoTech’s SuperMag Amine Beads are also available in an easy-to-use kit format to enable researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic beads. With the engineered SuperMag Amine Beads and the specifically formulated conjugation buffers, the resulting conjugates exhibit high binding capacity and low non-specific binding. The kit contains sufficient reagents & components for performing at least 4 conjugation reactions. Briefly, the magnetic beads are activated using SMCC (succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate) followed by conjugation to thiol groups that are present on the target protein/ligand. The recommended protocol has been used to successfully conjugate bovine serum albumin, streptavidin or immunoglobulin to these magnetic beads.

Features:

• High binding capacity
• Significantly low non-specific binding to proteins and nucleic acid
• Immediate and gentle coupling at room temperature and neutral to low pH
• Fast magnetic separation

Applications:

• CTC separation
• Cell separation
• Protein separation
• Bacteria separation
• Immunoassays