Product Introduction

Assay Protocol

 Routine PCR Protocol

1. Reaction setup:

We recommend assembling all reaction components on ice and quickly transferring the reactions to a thermocycler preheated to the denaturation temperature (95°C).

l It could be added to no more than 10% DMSO to the reaction system for high GC templates.

l The reaction volumes can be scaled up or down providing that the final concentrations of the components (DNA, dNTPs, labeled dNTP) are as indicated in the protocol.

2. Gently vortex the samples and spin down.

3. Perform PCR using the recommended thermal cycling conditions outlined below:

Note

1. It is recommended that thereaction system should be prepared on ice.

2. The optimization of PCR reactions can be adjusted from Mg2+ concentration, template amount, enzyme amount, annealing temperature, etc.

3. For your safety and health, pleasewearsafety glasses, gloves, or protective clothing.

For Research Use Only!