2×Fast Pfus PCR Master Mix (1 mL)

Description

This Fast Pfus PCR Master Mix is a 2×concantrated solution of optimized Pfu DNA polymerase, dNTPs and all other components required for PCR, expeceted DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps during PCR set up. The optimized Pfu DNA polymerase ensure effieicnt PCR reaction, high fidelity, and high specificity, and with quick annealing speed of 5-15s/kb.2×Fast sTaq PCR Master Mix ispremixed with DNA loading dye, so the obtained product can ber direct loading on gel.

Storage and Handling Conditions

Transport use wet ice. Store at 4℃for periods up to 12 months. For longer periods, stored at -20℃.

Assay Protocol

1.Gently vortex and briefly centrifuge PCR Master Mix(2×) after thawing.

2.Place a thin-walled PCR tube on ice and add the following components for each reaction accoridng to your desired reaction volume.

a:template quality is important for PCR reaction. Too little template result in low efficiency of PCR reaction, and excess template can result in nonspecific amplification. If the template is plasmid or from bacteriophage, 50ng-5pg added in 50μL rnxis recommended. If tempalted is genomic DNA, 250ng-50ng added in 50μL rnxis recommended. If template is cDNA, dilute your original cDNA by 2-100 times, and the volume of diluted cDNA added to reaction is no more than 10% of total reaction volume. If templtae is culture liquid, the volume added to reaction is no more than 10% of total reaction volume.

b:the appropriate primer concentration is between 0.2-1.0μM，and 0.4μM is recommended.

c: for high content GC template, DMSO with no more than 10% total volume can be added to improve PCR efficiency.

1.Gently vortex the samples and spin down.

2.When using a thermal cycler that does not contain a heated lid, overlay the reaction mixture with 20μL mirenal oil.

3.Perform PCR using the recommened thermal cycling conditions below:

Step	Temperature	Time	Cycles
Initial Denaturationa	98℃ Write a review Your Name Your Review Note: HTML is not translated! Rating Bad Good Continue Thumb File information G3305-01 2Fast Pfus PCR Master Mix.pdf File Size245.87KB Downloaded: 35 You Might Like From the Same Brand Quickview New 2 × Fast SYBR Green qPCR Master Mix (High ROX) 1mL Product Description/Introduction2 × Fast SYBR Green qPCR Master Mix (High ROX) is a special 2× premix for qPCR reaction using SYBR Green I chimeric fl.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 2 × Fast SYBR Green qPCR Master Mix (Low ROX) 1mL Product Description/Introduction2× Fast SYBR Green qPCR Master Mix (Low ROX) is a special 2× premix for qPCR reaction using SYBR Green I chimeric fluo.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 2 × Fast SYBR Green qPCR Master Mix (None ROX) 1mL Product Description/Introduction2× Fast SYBR Green qPCR Master Mix (None ROX) is a special 2× premix for qPCR reaction using SYBR Green I chimeri.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 2 × SYBR Green qPCR Master Mix (High ROX) 1mL Product Description/Introduction2×SYBR Green qPCR Master Mix (High ROX) is a ready-to-use solution optimized for quantitative real-time PCR and t.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 2 × SYBR Green qPCR Master Mix (None ROX) 1mL Product Description/Introduction2×SYBR Green qPCR Master Mix (None ROX) is a special 2× premix for qPCR reaction using SYBR Green I chimeric fluoresce.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview 2×Fast sTaq PCR Master Mix (1 mL) .. $20.00 Ex Tax:$20.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview 0.9% NaCl 0.9% NaCl.. $17.00 Ex Tax:$17.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 1L PES Membrane Vacuum Filtration System (0.22 uM) 1000ml PES membrane Vacuum Filtration Systems­­­­­­­­­­­­­­­­­­­­­­­­­­­­­ With ISO9001&13485 certified, 1000ml PES membrane Vacuum Filtratio.. $243.00 Ex Tax:$243.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 2 × Fast SYBR Green qPCR Master Mix (High ROX) 1mL Product Description/Introduction2 × Fast SYBR Green qPCR Master Mix (High ROX) is a special 2× premix for qPCR reaction using SYBR Green I chimeric fl.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 2 × Fast SYBR Green qPCR Master Mix (Low ROX) 1mL Product Description/Introduction2× Fast SYBR Green qPCR Master Mix (Low ROX) is a special 2× premix for qPCR reaction using SYBR Green I chimeric fluo.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 2 × Fast SYBR Green qPCR Master Mix (None ROX) 1mL Product Description/Introduction2× Fast SYBR Green qPCR Master Mix (None ROX) is a special 2× premix for qPCR reaction using SYBR Green I chimeri.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question Quickview New 2 × SYBR Green qPCR Master Mix (High ROX) 1mL Product Description/Introduction2×SYBR Green qPCR Master Mix (High ROX) is a ready-to-use solution optimized for quantitative real-time PCR and t.. $89.00 Ex Tax:$89.00 Add to Cart Add to Wish List Compare this Product Buy Now Ask Question