KeyTec® Renilla Luciferase Detection Kit

Overview

KeyTec® Renilla luciferase Detection kit is designed for the sensitive and robust detection of Renilla luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Renilla luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, Coelenterazine reacts with Renilla luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Renilla luciferase reporter gene expression in mammalian cells.

Performance

Use KeyTec® Renilla Luciferase Detection Kit to detect the overexpression of Renilla luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Components

Notices

Certificate of Analysis	Limitations	Storage Conditions
	For research use only	-20 ℃