KeyTec® Enhanced Luciferase Detection Kit (no DTT)

Overview

KeyTec® Enhanced Luciferase Detection Kit (no DTT) is designed for the sensitive and robust detection of Firefly luciferase reporter gene assays without Dithiothreitol. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.

Performance

Use KeyTec® Enhanced Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Components

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-40 ℃