KeyTec® cAMP TR-FRET KIT (10000 tests)
- Stock: In Stock
- Model: 1143
Overview
KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.
Components
| CAT. | Description | Size |
| 1143 | KeyTec® cAMP TR-FRET KIT | 1000 tests |
| The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 1 × 50 µL mAb anti-cAMP-LA (100X) • 1 × 25 μL Biotin-cAMP (200X) • 1 × 100 µL cAMP Standard Solution (50 μM) • 1 × 25 μL Streptavidin-Solar Eu (200X) • 2 × 1 mL Detection Buffer (5X) |
||
Notices
| Certificate of Analysis | Limitations | Storage Conditions |
| For research use only | 
-60℃ or below |
| Thumb | File information |
 |
ProtocolKeyTeccAMPTR-FRETKIT.pdf File Size988.72KB Downloaded: 6 |
1-250x250.jpeg "2 × Fast SYBR Green qPCR Master Mix (High ROX) 1mL")
1-250x250.jpeg "2 × Fast SYBR Green qPCR Master Mix (Low ROX) 1mL")