EndoFree Maxi Plasmid Kit

Name: EndoFree Maxi Plasmid Kit
Brand: Tiangen
Price: 198.00 USD
Availability: InStock

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Storage
Room temperature (15-25℃ )
Description
The EndoFree Maxi Plasmid Kit is based on silica membrane technology to extract endotoxin-free plasmid DNA. This kit provides a unique endotoxin removal process to generate high-quality plasmid DNA for downstream applications. It is designed to isolate plasmid DNA of the highest purity from 100-200 ml of bacterial culture and could yield up to 1.5 mg transfection grade plasmid DNA.
Features
■ Fast and high yield: 200 μg-1.5 mg plasmid DNA yield in 1 hour with high proportion of super-coiled structure.
■ High-purity: High-purity plasmid DNA are acquired by unique buffer system and Spin Column CP6.
■ Excellent transfection efficiency: Suitable for transfection experiments of most cell lines.
■ Wide range of applications: Suitable for restriction enzyme digestion, transformation, sequencing, microinjection, gene silencing and transfection experiments.

Experimental Example

High/low copy plasmid extraction
Low-copy plasmid pBR322, extracted from 200 ml bacterial culture using EndoFree Maxi Plasmid Kit, was eluted in 1 ml Buffer TB, with a concentration of 0.6 μg/μl. High-copy plasmid pBS, extracted from 100 ml bacterial culture using EndoFree Maxi Plasmid Kit ,was eluted in 1 ml Buffer TB, with a concentration of 1.2 μg/μl.
MIV: TIANGEN DNA Marker IV
pBR322: 2 μl, pBS : 2 μl