Anti -GAPDH Rabbit pAb 100 μL
- Stock: In Stock
- Model: 0463
Product Information
Protein full name | Actin, cytoplasmic 1 |
Synonyms | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse beta-Actin |
Uniprot ID | P60710 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 45 kDa |
Applications
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | spleen, kidney, brain |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 2000 | colon, kidney, liver |
ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549 |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
 | WB analysis of beta Actin (GB12001). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
| Immunohistochemistry of paraffin embedded human colon using actin (GB12001) at dilution of 1: 1000 (200x lens) |
 | Immunohistochemistry of paraffin embedded rat liver using actin (GB12001) at dilution of 1: 1000 (200x lens) |
 | Immunohistochemistry of paraffin embedded mouse stomach using actin (GB12001) at dilution of 1: 1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat kidney using actin (GB12001) at dilution of 1: 1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded human liver cancer using actin (GB12001) at dilution of 1: 1000 (200x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB12001) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB12001) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer.
1-250x250.jpeg "2 × Fast SYBR Green qPCR Master Mix (High ROX) 1mL")
1-250x250.jpeg "2 × Fast SYBR Green qPCR Master Mix (Low ROX) 1mL")
