Leibovitz's L-15 Medium

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration. This L-15 is modified as follows: With Without • Galactose • Glucose • Phenol Red • HEPES • L-glutamine • Sodium Bicarbonate • Sodium Pyruvate The complete formulation is available upon request. L-15 contains no proteins, lipids, or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).