One-Step DNA & RNA Removal Kit

Introduction

Hygia One-Step DNA & RNA Removal Kit uses endonuclease -immobilized magnetic beads to remove DNA and RNA from sample using a single step protocol. The recombinant endonuclease is encoded by the same gene of Merck Benzonase nuclease or TurboNuclease) of Serratia macescens produced in E. coli. This nuclease nonspecifically digests all kinds of DNA and RNA, including variants of single- and double-stranded, circular, linear, or supercoiled DNA and RNA to 5’-phosphorylated oligonucleotides of 2-8 bases in length and is free of protease activity. The nuclease immobilized magnetic bead can efficiently remove all the nucleic acids (DNA and RNA) from protein solution with no endonuclease remaining in the solution due to the nuclease stably and covalently conjugated with the magnetic Beads.

Applications

An immobilized nuclease bead is ideal for removing nucleic acid contamination from purified proteins or other sample preparations.

Features and Advantages

● Efficient one-tube and extraction-free protocol.

● Ultrafast: Process 96 samples in less than 30 minutes with <10-second Hands-on Time.

● Nuclease Recovered at the end of the reaction thereby can be reused.

● Easy separation of the endonuclease from the protein sample.

● Stability of the immobilized endonuclease increases.

● Cost-effective: Eliminates columns, filters, laborious, organic reagents, and minimal plasticware required.

● High throughput: Compatible with many different automated liquid handling systems.