Quick Albumin Removal Kit

Features and Advantages

●Quick, Easy, eliminates columns, centrifuge, or filters.

●Remove >95% Albumin

●High throughput: Rapidly process up to 96 samples in less than 30 minutes

●Low abundance enrichment equal to or better than hexapeptides or antibodies

●Mild elution preserves the tertiary structure and allows for easy transfer to secondary analysis

●Scalable – easily adjusts for sample size and automation

●Minimal sample dilution

●Suitable for LC-MS, activity-based protein profiling, and proteomic research

PROTOCOL

Note

●The following protocol is an example. Protein concentrations in serum might vary. Ten microliters of human serum have about 700µg of complete protein. About 70% of that will be human serum albumin. The amount of serum used in each response should be optimized by the user. Overloading the system may result in albumin carryover into the low abundant protein fraction. Using the given protocol, the user should expect a typical elution to include 50-100µg of serum protein.

●The samples are eluted in a salt-containing buffer and may need to be desalted before electrophoresis or mass spectrometry analysis. If the sample is sufficiently diluted before analysis, desalting may not be required.

Materials Required

●BcMag™ Quick Albumin Removal magnetic beads

●1x Binding/Washing Buffer: 20 mM sodium phosphate, pH 6.5

●1x Elution Buffer: 100 mM glycine-HCl, pH 2.7