One-Step Deproteinizing Sample Preparation

Introduction

The presence of protein and lipids in biological fluid samples frequently interferes with small molecule analysis, decreasing accuracy and sensitivity. Numerous bioassays need the removal of proteins (deproteinization) and lipids (delipidation) from samples before analysis, such as LC/MS/MS analysis.

Precipitation using trichloroacetic acid, perchloric acid, phosphotungstic acid, acetonitrile, methanol, and other organic solvents has been extensively used for deproteinization over the last half-century. However, these procedures are not only tedious, time-consuming, and challenging to adapt to automation but also inefficiently remove lipids and nucleic acids. 

Hygia One-Step Deproteinizing Kit provides a novel solution for the deproteinization, delipidation, and nucleic acid from biological fluid samples such as cell lysis, plasma, serum, tissue homogenate, and urine for analysis of a variety of small molecules. The superparamagnetic magnetic beads efficiently utilize the proprietary matrix to bind proteins and lipids in the sample. The one-minute protein removal protocol is straightforward: one step, one tube, no precipitation, no filtration, and no centrifugation. The resin enables 96 samples to be processed simultaneously in less than 10 minutes.

Features and Advantages

● Fast and straightforward protocol: one step, one tube, process 96 samples in less than 10 minutes

● Easy-to-use: Eliminates columns, filters, laborious repeat pipetting or centrifugation, and easily adjusts for sample size and automation

● No organic solvents/acids precipitation

● Efficient deproteinization