One-Step Single-Stranded DNA Removal Kit

Applications

● Presequencing clean-up for PCR products

● Megaprimer PCR for site-directed mutagenesis

● Removal of ssDNA containing a 3′-hydroxyl terminus from nucleic acid mixtures

● Assay for the presence of single-stranded DNA with a 3′-hydroxyl terminus

Features and Advantages

● Efficient one-tube and extraction-free protocol

● Ultrafast: Process 96 samples in less than 30 minutes with <10-second Hands-on Time

● Nuclease recovered at the end of the reaction thereby can be reused.

● Easy separation of the nuclease from the reaction.

● Stability of the immobilized nuclease increases.

● Cost-effective: Eliminates columns, filters, laborious, organic reagents, and minimal plasticware required.

● High-throughput: Compatible with many different automated liquid handling systems.