One-Step DNA & RNA Cleanup Kit
- Stock: In Stock
- Model: 0139
Available Options
Introduction
Hygia One-Step DNA & RNA Cleanup Kit provides one-step removal of impurity by negative chromatography from pre-purified samples of DNA/RNA. The impurity includes DNA/RNA polymerases, modifying enzymes, restriction endonucleases, ligases, kinases, nucleases, phosphatases, protein, most of the detergent, most of the fluorescent or no fluorescent dyes, divalent cations such as Ca2+, Mg2+, excess primer, dimer, adapter, DNA/RNA fragments (<100- mer ssDNA), free dNTPs/NTPs and as well as and their analogs including radiolabeled, biotinylated and fluorescent derivatives.
The protocol is straightforward: one tube, one step, and one minute. Added magnetic beads directly to the pre-purified DNA/RNA samples and vortex or pipette to capture and remove the impurities. After vortexing/pipetting, the beads are captured by a magnetic Rack, while the supernatant contains the purified and ready-to-run products. The beads are suitable for DNA/RNA fragments, plasmid DNA and genomic DNA.
Features and Advantages
● Simple protocol: No liquid transfer, One-tube, One-step
● Ultrafast: One-minute protocol
● Higher purity and recovery > 90% DNA
● Effective Cleanup: Removes excess primer (<100- mer ssDNA), dimer, adapter, a salt such as Mg2+, detergent, dNTPs, enzymes, and dye
● Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and ethanol
● High throughput: Compatible with many different automated liquid handling systems