One-Step Universal Forensic Trace DNA Purification Kit

Introduction

HygiaReagents One-step DNA Purification System uses novel negative chromatography magnetic beads to quickly deliver higher quality and superior DNA yield from most trace forensic DNA samples. Those samples include body fluids, stains, swabs of body fluids, strip-removed cells, cigarette butts, Hair follicles, fingernail scrapings, epithelial cells, bite marks, semen, touch DNA samples, etc. The specially designed magnetic beads with our proprietary surface chemistry function simultaneously to lyse cells and capture the PCR inhibitors once mixed with the sample. The magnetic beads-PCR inhibitor complex was then magnetically removed by a magnet while the pure DNA remaining in the solution was ready for downstream STR analysis.

How It Works

1. Add functional magnetic beads to the sample.

2. Mix the samples with the magnetic beads and heat to lyse the cells.

3. Vortex for 5 minutes to capture the PCR inhibitors.

4. Remove the beads with a magnet.

5. Aspirate the supernatant containing the pure ready-to-use DNA.

· Blood & Stain

· Buccal Mucosa

· Nasal swab

· Throat swab

· Touch DNA Samples

· Epithelial Cells

· Bite Marks

· Body Fluids

· Cigarette Butts

· Hair Follicles

· Fingernail Scrapings

Performance

The purified DNA is suitable for use in sensitive downstream applications, such as PCR, qPCR, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, genotyping, or next-generation sequencing (NGS), buccal DNA sample collection, veterinary genotyping and diagnostics, research genotyping, Veterinary genotyping and diagnostics, genetic testing, forensics, population studies.

Features and Advantages

● Consistent accuracy, reproducibility, and sensitivity with a trace amount of sample.

● DNA extraction-free

● One-Step, One-Tube, No liquid transfer.

● No DNA damage, loss, and higher yields of higher-purity.

● Process hundreds of samples in less than 30 minutes.

● Effectively removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc. (See picture “PCR inhibitor removal”).

● Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.

● High-throughput: Compatible with many different automated liquid handling systems.