IntroductionThe Significance of Hair in Forensics and Medical ResearchHair is one of the most commonly found evidence at crime scenes and is gaining importance as a sample type for medical research inquiries. In criminology, hair has been used for population-based statistical analysis and DNA-based ..
IntroductionSexual assault cases (also called Rape cases) are some of the most challenging and sensitive cases for forensic scientists and law enforcement. Sexual assault cases require a clear male DNA profile in order to analyze autosomal DNA. However, mixed DNA samples collected from female victim..
IntroductionBiotin, also referred to as vitamin H, is a small molecule that is not commonly found in biological samples. It has a remarkable binding affinity for avidin (Ka=10^15) and streptavidin (Ka=10^14), making biotinylated molecules such as proteins or other molecules very useful for non-radio..
IntroductionPCR product purification is essential for successful downstream applications by removing enzymes, nucleotides, primers, and buffer components. There are several techniques for PCR product purification, including precipitation with PEG or phenol-chloroform extraction followed by ethanol p..
IntroductionPCR technology has become one of the most potent molecular biological tools in the last few decades. However, biological samples collected from different materials often contain various PCR inhibitors that can interfere with PCR amplification. PCR inhibitors are a diverse group of compou..
IntroductionSanger sequencing, also known as “chain terminator sequencing,” is a method of DNA sequencing that relies on DNA polymerase’s selective incorporation of fluorescent-labeled (bigdye terminator) dideoxy nucleotide chain terminators during in vitro DNA replication for use in a single sequen..
IntroductionSodium dodecyl sulfate is one of the most used detergents for solubilizing biological materials. Still, excess unbound detergent interferes with many downstream applications like mass spectrometry (MS) and amino acid sequencing, antigen-antibody binding, immunoprecipitation assay, and EL..
Applications● Presequencing clean-up for PCR products● Megaprimer PCR for site-directed mutagenesis● Removal of ssDNA containing a 3′-hydroxyl terminus from nucleic acid mixtures● Assay for the presence of single-stranded DNA with a 3′-hydroxyl terminusFeatures and Advantages● Efficient one-tube and..
IntroductionHygiaReagents One-step DNA Purification System uses novel negative chromatography magnetic beads to quickly deliver higher quality and superior DNA yield from most trace forensic DNA samples. Those samples include body fluids, stains, swabs of body fluids, strip-removed cells, cigarette ..
IntroductionHuman serum or plasma contains a wealth of proteomic information and is frequently analyzed for protein biomarkers of normal and pathological states. The extensive concentration range of proteins contained in human serum is one of the most challenging obstacles in studying it. Proteins t..
Features and Advantages●Quick, Easy, eliminates columns, centrifuge, or filters.●Remove >95% Albumin●High throughput: Rapidly process up to 96 samples in less than 30 minutes●Low abundance enrichment equal to or better than hexapeptides or antibodies●Mild elution preserves the tertiary structure ..
IntroductionEndotoxins, also known as Lipopolysaccharides (LPS), are a type of pyrogen with large complex molecules. They consist of an innermost core of hydrophobic fatty acid groups and a central and outermost region composed of hydrophilic polysaccharides. They are part of the outer membrane of t..