One-Step Detergent Removal Kit

Introduction

Detergents contain a hydrophilic head group and a hydrophobic tail. The hydrophobic moiety usually consists of a hydrocarbon chain, while the hydrophilic part has a polar head. Three types of detergents are commonly used in laboratory research, including:

● Non-ionic detergents contain uncharged hydrophilic head groups such as Triton X-100, Triton X-114, NP-40, Tween-20, and Tween-80.

● Ionic detergents are comprised of a hydrophobic chain and anionic or cationic head groups such as sodium dodecyl sulfate (SDS) and cetyltrimethylammonium bromide (CTAB).

● Zwitterionic detergents: Those detergents like CHAPS contain negatively and positively charged atomic groups in equal numbers. Therefore, they do not possess a net charge.

Detergents (surfactants) are essential for biomedical research. For successful downstream analysis, it is critical to reduce or entirely remove unbound detergents from the biological sample. However, excess unbound detergent is usually poorly compatible with many downstream applications, including ELISA, protease digestion of proteins, isoelectric focusing, and mass spectrometry (MS). Several commercially available detergent removal methods include prolonged dialysis, anion exchange chromatography, detergent removal spin column, and acetone precipitation. However, these procedures, such as using detergent removal columns, are either laborious or suffer from sample losses and are challenging for low volume samples and high thorough-put automation. We developed a novel, efficient surfactant removal system to overcome these limitations.

Hygia One-Step Detergent Removal Kit uses magnetic resin coated with proprietary chemistry to remove detergents. Compared with the detergent removal columns, the resin can quickly and efficiently remove free detergents from the sample with just a single step and enables individual samples or 96 samples to be processed simultaneously in less than 1 minute or 10 minutes with 95% sample recovery. Since the magnetic resin only adsorbs the detergent, the sample recovery rate is exceptional >90%-95%.

Features and Advantages

● Simple protocol: No liquid transfer, One-tube, One-step, and one-minute protocol

● Easy-to-use

● Reliable and reproducible results with exceptional >90% recovery for protein (>6 kDa, aprotinin) or DNA/RNA (>25mer dsDNA)

● Effective Cleanup: Remove 95% free detergent.

● Cost-effective: Eliminates columns, filters, and laborious repeat pipetting

● High throughput: Compatible with many different automated liquid handling systems