IntroductionPCR product purification is essential for successful downstream applications by removing enzymes, nucleotides, primers, and buffer components. There are several techniques for PCR product purification, including precipitation with PEG or phenol-chloroform extraction followed by ethanol p..
IntroductionPCR technology has become one of the most potent molecular biological tools in the last few decades. However, biological samples collected from different materials often contain various PCR inhibitors that can interfere with PCR amplification. PCR inhibitors are a diverse group of compou..
IntroductionSanger sequencing, also known as “chain terminator sequencing,” is a method of DNA sequencing that relies on DNA polymerase’s selective incorporation of fluorescent-labeled (bigdye terminator) dideoxy nucleotide chain terminators during in vitro DNA replication for use in a single sequen..
IntroductionSodium dodecyl sulfate is one of the most used detergents for solubilizing biological materials. Still, excess unbound detergent interferes with many downstream applications like mass spectrometry (MS) and amino acid sequencing, antigen-antibody binding, immunoprecipitation assay, and EL..
Applications● Presequencing clean-up for PCR products● Megaprimer PCR for site-directed mutagenesis● Removal of ssDNA containing a 3′-hydroxyl terminus from nucleic acid mixtures● Assay for the presence of single-stranded DNA with a 3′-hydroxyl terminusFeatures and Advantages● Efficient one-tube and..
IntroductionHygiaReagents One-step DNA Purification System uses novel negative chromatography magnetic beads to quickly deliver higher quality and superior DNA yield from most trace forensic DNA samples. Those samples include body fluids, stains, swabs of body fluids, strip-removed cells, cigarette ..
Product DescriptionInnovative rolling design is adopted for Automatic Film Sealer, which can effectively avoid sample loss and spatial cross-infection. It can be applied in conventional PCRfPolymerase Chain Reaction) and fluorescence QPCR(Qualitative Polymerase Chain Reaction). The machine is easy f..