AmeriDx Oligo Antibody Conjugation Kit

Background Introduction

The Oligo-Antibody Conjugation Kitrevolutionizes the way researchers create custom conjugates for advanced molecular biology applications. Leveraging bioorthogonal chemistry (click chemistry), this kit enables rapid, efficient, and site-specific conjugation of amine-modified oligonucleotides to antibodies. Designed for ease of use, the kit allows researchers to perform conjugations in their own lab, saving time and resources while maintaining high reproducibility.

Whether you're developing therapeutic antibodies, diagnostic probes, or research tools, this kit provides the reagents and controls needed to achieve reliable, high-quality conjugates.

Kit Components (Product Insert)

Each kit contains the following components:

1. Oligo Activation Reagent (5x 0.1 mg/vial, lyophilized).
2. Antibody Activation Reagent (5x 0.1 mg/vial, lyophilized).
3. Conjugation Buffer, 10 mL.
4. Amine Oligo Control: 1 nmol amine-modified oligo (lyophilized).
5. Antibody Control: 100 µg IgG (lyophilized).

Functionality

The kit utilizes click chemistry, a highly efficient and bioorthogonal reaction, to conjugate amine-modified oligonucleotides to antibodies. Here’s how it works:

1. Antibody Activation: Antibody activation reagent reacts with primary amines on the antibody.
2. Oligo Activation: Oligo activation reagent reacts with amine-modified oligos, purify activate oligo with AmeriDx(R) oligo & concentrator kit.
3. Conjugation: Mix the activated antibodies and oligo, initiate the specific click reaction to form stable conjugates.

Applications

The Oligo-Antibody Conjugation Kit is ideal for a wide range of applications, including:

• Therapeutic Development: Create antibody-Nucleic acid conjugates (ANCs) for targeted therapies.
• Diagnostics: Develop probes for qPCR, FISH, or sequencing applications.
• Research Tools: Generate custom conjugates for imaging, flow cytometry, or protein-DNA interaction studies.
• CRISPR and Gene Editing: Conjugate antibodies to guide RNAs or other oligonucleotides for targeted delivery.

Key Features

• High Efficiency: Achieve >90% conjugation efficiency with minimal side reactions.
• Rapid Reaction: Conjugation occurs in 1–2 hours at room temperature.
• User-Friendly: Includes detailed protocols, controls, and pre-measured reagents.
• Versatile: Compatible with a wide range of antibodies and amine-modified oligos.

Qualifications

• Quality Control: All reagents are rigorously tested for purity and functionality.
  • Purity: >95% by HPLC.
  • Functionality: Validated using control oligos and antibodies.
• Stability: Lyophilized reagents are stable for 12 months at -20°C.
• Scalability: Suitable for small-scale research or large-scale production.

Why Choose Our Kit?

• Reliability: Consistent performance across multiple applications.
• Expert Support: Access to technical support and detailed protocols.
• Cost-Effective: Eliminate the need for expensive custom conjugation services.